Module 2 Cell Biology 2
Section 2.1 DNA Replication
2.1.A: DNA/RNA Structure and Function
Mader Essential Study Partner Version 2—Genetics
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/004/index.html
Go to the Mader Online Learning Centre’s Essential Study Partner Version 2—Genetics and read the section entitled “DNA Structure.”
2.1.B: DNA Replication
Mader Essential Study Partner Version 2
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/001/index.html
Section 2.9 (Mitosis) offers a review of mitosis. View the animated film clip to see the process in action. As you watch, be sure you understand why DNA replication must precede mitosis.
Cell cycle animated diagram
Cells Alive: http://www.cellsalive.com/cell_cycle.htm
View a short animation of the cell cycle and note where DNA replication and mitosis fit into the cycle.
DNA Workshop
http://www.pbs.org/wgbh/aso/tryit/dna/#
Choose DNA Replication and follow the interactive instructions to replicate DNA.
Kyrk, John. Cell Biology Animation
http://www.johnkyrk.com/mitosis.html
Select DNA Replication and click on the green arrows to see a step-by-step animation of replication. Some terms (RNA primase, Okazaki fragments, and exonuclease) go beyond what you are expected to know. Focus on what is happening to the DNA strand in each step.
2.1.C: Recombinant DNA
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/index.html.
Find the section on “Recombinant DNA” and focus on “Technology” (sections 2.126–2.129) and “Applications” (sections 2.140–2.143).
Recombinant DNA: How Genes Are Transferred
http://www3.iptv.org/EXPLOREMORE/ge/what/recombinant.cfm.
Move your mouse over each picture to see how recombinant DNA is made. Click on each diagram to read the steps and view simple animations.
Explore More Genetic Engineering
http://www3.iptv.org/exploremore/ge/
This site contains useful, readable information on Genetic Engineering. Focus on “Recombinant DNA: How Genes Are Transferred.”
http://www.mhhe.com/biosci/genbio/olc_linkedcontent/bioethics_cases/index.html
If you’d like to dig into this topic a little more check out Case Study 1—Embryonic Stem Cells and Case Study 3—Cloning.
Section 2.2 Protein Synthesis
2.2.A: Protein Synthesis Part 1—Transcription
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/005/index.html
Read “Gene Activity” (2.100–Central Dogma) and “Transcription” (2.102–2.104) for an overview of concepts related to this lesson.
Cell Biology Animation
http://www.johnkyrk.com/DNAtranscription.html
Useful for helping you visualize transcription; can be viewed after this lesson or the next one.
DNA Workshop Activity
http://www.pbs.org/wgbh/aso/tryit/dna/#
A quick and easy interactive simulation of protein synthesis; you does the transcription and translation! This is an excellent tool to help visualize the process of creating a protein.
Transcribe and Translate a Gene
http://learn.genetics.utah.edu/content/molecules/transcribe/
For this interactive create-a-protein activity, where you are given a DNA strand to transcribe and translate. This works well as a review, and will help you visualize the two-step process of protein synthesis.
2.2.B: Protein Synthesis, Part 2: Translation
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/005/index.html
Read the section titled “Translation” (2.105 to 2.111). The animations for translation (2.108) and stages of translation (2.111) may be particularly useful.
Animation: Protein Synthesis
http://highered.mcgraw-hill.com/sites/0072421975/student_view0/chapter24/animations__english_.html#
An excellent rendition of the complex process of translation. Note that you are not responsible for knowing the names of the 70S ribosome or the P and A sites referred to in the clip.
Cell Biology Animation
http://www.johnkyrk.com/DNAtranslation.html
Watch the animation of translation. It can be helpful to actually see the process take place. The clip can be paused as needed.
DNA Workshop Activity
http://www.pbs.org/wgbh/aso/tryit/dna/index.html#
An interactive simulation of protein synthesis.
Transcribe and Translate a Gene
http://learn.genetics.utah.edu/content/molecules/transcribe/
For this interactive create-a-protein activity, you are given a DNA strand to transcribe and translate. This works well as a review, and will help you visualize the two-step process of protein synthesis. The activity is also offered as preamble to the DNA Microarray online experiment.
2.2.C: Mutations of DNA
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/005/index.html
In ‘Genetics → Protein Synthesis → Translation’ - Sections 2.112 and 2.113
Mutations are relevant to this lesson. In addition to reading these two short sections, watch the animation provided.
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/genetics/004/index.html
Genetics → DNA → DNA Mutation → Section 2.96 features an animated/narrated clip showing examples of point mutations (substitution) and frameshift mutations (insertion and deletion).
Section Assignment 2.2 Part E
If you’d like to try concept-mapping software, do an Internet search for “concept maps.” Numerous sites are devoted to this type of organizer. The following sites may also be helpful:
Inspiration Software
http://www.inspiration.com/vlearning/index.cfm?fuseaction=concept_maps
Inspiration software provides a free 30-day trial version of concept mapping software, along with samples and other graphic organizer ideas.
The Concept Mapping Homepage
http://users.edte.utwente.nl/lanzing/cm_home.htm
Scroll down to see a sample concept map and a list of links to downloadable software. If you decide to use a software program, select one that is free or that offers a free time-limited trial.
You’ll find a sample concept map @ http://users.edte.utwente.nl/lanzing/cm_home.htm This map demonstrates one possible way to connect several of the terms you learned in an earlier lesson on DNA.
Section 2.3 Enzymes
2.3.A: Enzyme Metabolism
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/cells/004/index.html
1.98 Introduction
1.99 Metabolic pathway—click and drag terms to the correct location on the enzyme-substrate diagram
1.100 Enzymes as Catalysts—narrated animation in 3-D
1.101 Pathways
1.102 Feedback Inhibition
To review what you’ve learned work through these sections and try dragging the labels to their correct locations on the diagram.
Mader Online
http://highered.mcgraw-hill.com/sites/0072437316/student_view0/chapter8/animations.html#
Watch the animated clip titled “Feedback Inhibition of Biochemical Pathways.” In addition to the Mader Online reference 1.102 above, this offers another example of feedback inhibition (competitive).
QuizmeBC—Provincial Exam Preparation
http://www.quizmebc.ca/quiz.php
If you feel you need more practice, visit “QuizmeBC,” an online provincial exam preparation resource. Choose “Cell processes and applications” to generate a practice quiz. You can answer only those questions that apply to what you’ve learned so far, and you will receive immediate feedback. The questions are taken from past exams, so they give a good sense of what to expect when you write the provincial exam.
2.3.B: Factors Affecting Enzyme Action
Mader Online
http://www.mhhe.com/biosci/genbio/espv2/data/cells/004/index.html
Read the Introduction (Section 1.101) and watch Section 1.102 on Feedback inhibition (narrated RealPlayer animation in 3D).
Clinton Community College site—General Biology I
http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio%20101/Bio%20101%20Laboratory/Enzymes/Enzymes.htm
Offers an enzyme lab that uses readily attainable materials to investigate some of the factors affecting enzymatic reaction rates. The site also contains a blank data sheet and full instructions.
In addition, Mader Online Learning Centre Virtual Lab offers “Enzyme controlled reactions.”
http://www.mhhe.com/biosci/genbio/virtual_labs/BL_11/BL_11.html
This is a narrated animation of the formation of an ES complex followed by interactive experimentation with pH and substrate levels. You will table and graph results, then answer journal questions.
2.3.C: Thyroxin
Mader Online:
Animals—Endocrine System
http://www.mhhe.com/biosci/genbio/espv2/data/animals/004/index.html
Read the following section:
6.99 (Thyroid gland): Roll over the diagram to highlight features. This is a useful summary of the inter-relationship between the thyroid, hypothalamus, and anterior pituitary.
| 
